wyatt dawn 8 multiangle light scattering detector Search Results


superdex  (GE Healthcare)
98
GE Healthcare superdex
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Superdex, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superdex/product/GE Healthcare
Average 98 stars, based on 1 article reviews
superdex - by Bioz Stars, 2026-03
98/100 stars
  Buy from Supplier
dawn 8 heleos ii multiangle scattered light photometer  (Waters Corporation)
95
Waters Corporation dawn 8 heleos ii multiangle scattered light photometer
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Dawn 8 Heleos Ii Multiangle Scattered Light Photometer, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dawn 8 heleos ii multiangle scattered light photometer/product/Waters Corporation
Average 95 stars, based on 1 article reviews
dawn 8 heleos ii multiangle scattered light photometer - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
dawn 8+ multiangle light-scattering unit  (Waters Corporation)
90
Waters Corporation dawn 8+ multiangle light-scattering unit
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Dawn 8+ Multiangle Light Scattering Unit, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dawn 8+ multiangle light-scattering unit/product/Waters Corporation
Average 90 stars, based on 1 article reviews
dawn 8+ multiangle light-scattering unit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
dawn 8 multiangle laser light  (Waters Corporation)
96
Waters Corporation dawn 8 multiangle laser light
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Dawn 8 Multiangle Laser Light, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dawn 8 multiangle laser light/product/Waters Corporation
Average 96 stars, based on 1 article reviews
dawn 8 multiangle laser light - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
dawn  (Waters Corporation)
99
Waters Corporation dawn
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Dawn, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dawn/product/Waters Corporation
Average 99 stars, based on 1 article reviews
dawn - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
optilab  (Waters Corporation)
99
Waters Corporation optilab
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Optilab, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/optilab/product/Waters Corporation
Average 99 stars, based on 1 article reviews
optilab - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
size-exclusion chromatograph equipped with multiangle light scattering detectors dawn 8  (Waters Corporation)
90
Waters Corporation size-exclusion chromatograph equipped with multiangle light scattering detectors dawn 8
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Size Exclusion Chromatograph Equipped With Multiangle Light Scattering Detectors Dawn 8, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/size-exclusion chromatograph equipped with multiangle light scattering detectors dawn 8/product/Waters Corporation
Average 90 stars, based on 1 article reviews
size-exclusion chromatograph equipped with multiangle light scattering detectors dawn 8 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
1260 uv  (Agilent technologies)
90
Agilent technologies 1260 uv
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
1260 Uv, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1260 uv/product/Agilent technologies
Average 90 stars, based on 1 article reviews
1260 uv - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
multiangle light scattering detector  (Waters Corporation)
90
Waters Corporation multiangle light scattering detector
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Multiangle Light Scattering Detector, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multiangle light scattering detector/product/Waters Corporation
Average 90 stars, based on 1 article reviews
multiangle light scattering detector - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
polargel m columns  (Agilent technologies)
90
Agilent technologies polargel m columns
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Polargel M Columns, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polargel m columns/product/Agilent technologies
Average 90 stars, based on 1 article reviews
polargel m columns - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
dynamic light scattering detector wyatt-qels  (Waters Corporation)
90
Waters Corporation dynamic light scattering detector wyatt-qels
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Dynamic Light Scattering Detector Wyatt Qels, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dynamic light scattering detector wyatt-qels/product/Waters Corporation
Average 90 stars, based on 1 article reviews
dynamic light scattering detector wyatt-qels - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
uv/vis detector spd-20a  (Postnova Analytics)
90
Postnova Analytics uv/vis detector spd-20a
Structural characterisation <t>of</t> <t>TRAF6</t> in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a <t>Superdex</t> 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
Uv/Vis Detector Spd 20a, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uv/vis detector spd-20a/product/Postnova Analytics
Average 90 stars, based on 1 article reviews
uv/vis detector spd-20a - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Structural characterisation of TRAF6 in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a Superdex 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic

Journal: Nature Communications

Article Title: The activity of TRAF RING homo- and heterodimers is regulated by zinc finger 1

doi: 10.1038/s41467-017-01665-3

Figure Lengend Snippet: Structural characterisation of TRAF6 in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ 3 and RZ 1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ 3 TRAF6. Each form of TRAF6 RZ 3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 o C for the indicated times. c Analytical size exclusion profile of 250 µM Dr RZ 3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a Superdex 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of Dr RZ 3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic

Article Snippet: For determination of the oligomeric status of TRAF6, multiangle light scattering (MALS; Dawn 8+, Wyatt) coupled to a Superdex 200 10/300 GL (GE) was used.

Techniques: Construct, Activity Assay, Incubation, SDS Page